Study of Developmental Stages of Methylosinus trichosporium with the Aid of Fluorescent-Antibody Staining Techniques.

نویسندگان

  • W M Reed
  • P R Dugan
چکیده

When stained by using an indirect fluorescent-antibody technique, Methylosinus trichosporium displayed an uneven fluorescence. Exospores and the polar tips of some vegetative cells displayed a more intense fluorescence than the other cells. Cross-absorption of the specific anti-M. trichosporium immunoglobulin G with exospores resulted in no fluorescence of exospores or exospore regions of sporulating vegetative cells. This demonstrated that antigens were present on exospores and exospore regions of vegetative cells that are different from vegetative cell antigens. Taking advantage of this phenomenon, three fluorescentantibody staining techniques were developed which were used to study the life cycle of M. trichosporium.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Poly-β-hydroxybutyrate Production by Methylosinus trichosporium OB3b at Different Gas-phase Conditions

Background: The utilization of methane for production of Poly-β-hydroxybutyrate (PHB) not only cuts the emissions of greenhouse gases but also greatly reduces PHB production cost. Objectives: The aim of this study was to determine the effects of gas-phase conditions on PHB production by Methylosinus trichosporium OB3b. Ma...

متن کامل

The Completed PacBio Single-Molecule Real-Time Sequence of Methylosinus trichosporium Strain OB3b Reveals the Presence of a Third Large Plasmid

Presented here is the complete genome sequence of the well-studied Rhizobiales methanotroph Methylosinus trichosporium strain OB3b. The assembly contains 5,183,433 bp, corresponding to a chromosome of 4,508,832 bp and three circular plasmids of 285,280 bp, 209,102 bp, and 180,219 bp.

متن کامل

Purification and physical-chemical properties of methanobactin: a chalkophore from Methylosinus trichosporium OB3b.

Methanobactin is an extracellular, copper-binding chromopeptide from the methane-oxidizing bacterium, Methylosinus trichosporium OB3b, believed to be involved in copper detoxification, sequestration, and uptake. Although small (1217.2 Da), methanobactin possesses a complex three-dimensional macrocyclic structure with several unusual moieties. The molecule binds one copper and has the N-2-isopro...

متن کامل

Effect of Chlorinated Ethene Conversion on Viability and Activity of Methylosinus trichosporium OB3b.

The effect of transformation of chlorinated ethenes on the cell viability of Methylosinus trichosporium OB3b was investigated. A comparison of the loss of viability with the decrease in transformation rates showed that for the monooxygenase-mediated transformation of all chlorinated ethenes except vinyl chloride the decrease in cell viability was the predominant toxic effect.

متن کامل

Characterization of a methane-utilizing bacterium from a bacterial consortium that rapidly degrades trichloroethylene and chloroform.

A mixed culture of bacteria grown in a bioreactor with methane as a carbon and energy source rapidly oxidized trichloroethylene and chloroform. The most abundant organism was a crescent-shaped bacterium that bound the fluorescent oligonucleotide signature probes that specifically hybridize to serine pathway methylotrophs. The 5S rRNA from this bacterium was found to be 93.5% homologous to the M...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 38 6  شماره 

صفحات  -

تاریخ انتشار 1979